Characterization of Candidate Live Oral Salmonella typhi Vaccine Strains Harboring Defined Mutations in aroA , aroC , and htrA
نویسندگان
چکیده
منابع مشابه
Evaluation in volunteers of a candidate live oral attenuated Salmonella typhi vector vaccine.
Candidate vector vaccine strain CVD 906 (aroC- and aroD- derivative of virulent Salmonella typhi strain ISP1820) was evaluated in phase 1 clinical trials. The first nine volunteers ingested a single dose of 5 x 10(7) CVD 906 bacilli. At this dose CVD 906 stimulates remarkable systemic and mucosal immune responses, inasmuch as 89% of volunteers developed marked serum antibody levels to S. typhi ...
متن کاملOptimization of plasmid maintenance in the attenuated live vector vaccine strain Salmonella typhi CVD 908-htrA.
The broad objective of the research presented here is to develop a noncatalytic plasmid maintenance system for the stabilization of multicopy expression plasmids encoding foreign antigens in a Salmonella typhi live-vector vaccine strain such as CVD 908-htrA. We have enhanced the maintenance of expression plasmids at two independent levels. First, we removed dependence upon balanced-lethal maint...
متن کاملPhase 2 clinical trial of attenuated Salmonella enterica serovar typhi oral live vector vaccine CVD 908-htrA in U.S. volunteers.
Salmonella enterica serovar Typhi strain CVD 908-htrA is a live attenuated strain which may be useful as an improved oral typhoid vaccine and as a vector for cloned genes of other pathogens. We conducted a phase 2 trial in which 80 healthy adults received one of two dosage levels of CVD 908-htrA in a double-blind, placebo-controlled, crossover study. There were no differences in the rates of si...
متن کاملComparison of abilities of Salmonella enterica serovar typhimurium aroA aroD and aroA htrA mutants to act as live vectors.
We compared the ability of Salmonella enterica serovar Typhimurium SL1344 aroA aroD (BRD509) and aroA htrA (BRD807) mutants to act as live vectors for delivery of fragment C of tetanus toxin (FrgC). FrgC was expressed in these strains from either pTETnir15 or pTEThtrA1. BRD509FrgC(+) strains elicited approximately 2-log-higher serum anti-FrgC antibody titers than BRD807FrgC(+) strains. All mice...
متن کاملEffect of aroA, htrA and aroA-htrA Deletion Mutation on Ability of Salmonella Abortusequi to Survive and Multiply in Macrophages
To understand the problem of host specificity of Salmonella enterica subspecies enterica serovar Abortusequi (S. Abortusequi), five isogenic strains including aroA, htrA and aroA-htrA deletion mutants, virulence plasmid cured and wild type parent strains were tested for invasion, survival and multiplication (up to 30 h) in cattle, goat, buffalo, horse and guinea pig macrophages and murine macro...
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ژورنال
عنوان ژورنال: Infection and Immunity
سال: 1999
ISSN: 0019-9567,1098-5522
DOI: 10.1128/iai.67.2.700-707.1999